3x Flag Peptide

The 3x FLAG peptide is a multifunctional fusion tag for the purification of recombinant proteins. FLAG peptide maintances the natural folding of its fusing proteins. FLAG peptide can be removed by enterokinase, and eluted under non-denaturing conditions.

The FLAG peptide, with the sequence DYKDDDDK, is a short amino acid sequence that serves as a versatile tag for protein purification and detection. It is widely used in various applications such as affinity purification, immunoprecipitation, Western blotting, ELISA, and flow cytometry

3x FLAG peptide, also known as the DYKDDDDK peptide, offers several benefits in protein research, primarily in purification and detection. It's a short, hydrophilic peptide sequence that can be easily added to a protein of interest using recombinant DNA technology. This allows researchers to use FLAG antibodies for purification and detection of the tagged protein.

Highly Specific Purification:
FLAG tags allow for highly specific protein purification with minimal background noise.
Versatile Applications:
FLAG tags are compatible with various downstream applications, including Western blotting, immunoprecipitation, flow cytometry, and protein-protein interaction studies.
Mild Elution Conditions:
FLAG-tagged proteins can be eluted from antibody resins under relatively mild conditions, preserving the protein's native structure and function.
Cost-Effective:
FLAG tags are widely used, with many commercially available antibodies and reagents, making them a cost-effective option for protein purification.
Facilitates Study of Protein Complexes:
FLAG tags are particularly useful for studying protein complexes, as the mild purification process tends not to disrupt these interactions.
Enhanced Detection:
The 3xFLAG tag, which consists of three tandem FLAG epitopes, provides even greater sensitivity for detection and purification compared to the single FLAG tag.

Application

1. Protein Purification:
The FLAG tag allows for the efficient purification of proteins by binding to anti-FLAG antibodies immobilized on a resin, enabling the separation of the tagged protein from other cellular components.
The FLAG peptide can be used to elute the tagged protein from the affinity resin by competing with the antibody binding.
2. Detection and Analysis:
The FLAG tag facilitates the immunoprecipitation of proteins using anti-FLAG antibodies, allowing for their isolation and subsequent analysis.
The FLAG peptide can be used as a probe to detect the tagged protein in Western blots.
The FLAG tag can be used in ELISA and flow cytometry assays to detect and quantify the tagged protein.
3. Other Applications:
The 3x FLAG tag can be used in various cellular assays to study protein localization, interactions, and function.
The FLAG tag includes an enterokinase cleavage site, allowing for its removal after purification using enterokinase.

When using 3X labeled peptides, the following should be noted:

Storage:

3X-labeled peptides should be stored at -20 °C to avoid drying or freeze-thawing. It can also be stored at 2-8°C, but it is important to note that temperature changes may affect its stability.

Preparation before use:

The 3X-labeled peptide should be thoroughly resuspended before use and bubbles should be avoided. After resuspension, it should be quickly transferred to the EP tube to be used.

Avoid contamination:

When using 3X-labeled peptides, they should be protected from contamination such as bacteria, mycoplasma, etc. If contamination is found, stop using it immediately and take appropriate measures.

Correct use:

When using 3X labeled peptides, follow the operation steps on the instructions to avoid misoperation and lead to experimental failure or error.

Pay attention to the dosage:

When using 3X labeled peptides, an appropriate amount of labeled peptides should be used according to the experimental requirements and the recommendations on the reagent instructions to avoid wasting or affecting the experimental results.

Pay attention to storage conditions:

Unused 3X-labeled peptides should be re-stored at -20°C to avoid repeated freeze-thaw or drying.

In conclusion, attention should be paid to the storage and use method of 3X-labeled peptides to ensure the accuracy and reliability of experimental results. If you have any questions, it is advisable to consult an expert in the relevant field or refer to the reagent instructions.

Delivery

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≤50kg, Express delivery ( DHL / FEDEX ) recommended, usually called as DDU service.

≤500kg, Air shipping recommended, usually called as CIF service.

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For high value products, please select air shipping and express delivery for safe.

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Q1: Can l get some free sample for a test?

A:Yes, we can offer small quantity free sample for your test if you can pay for the shipping cost or freight collect.

Q2: What's your MOQ of 3x Flag Peptide?

A:Our minimum order quantity is 1 box. But usually we accept less quantity of some special and expensive products,you can contact us for more information.

Q3: How about delivery time?

A:Within 3~7 working days by Express.

Q4: ls there any discount?

A:Different quantity has different discount, the more you buy, the more you will save.

Q5: How do you treat quality complaint?

A:First of all, our quality control will reduce the the quality problem to zero. lf any questions, we will send you free goods again or fully refund you.

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